Protein_Domain

Part:BBa_K5466004:Design

Designed by: Adrián Gómez Lara, Daniel Bulnes Roldán   Group: iGEM24_UMA-MALAGA   (2024-09-22)


AntiAFB1- Nb28-S102D


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Nb28 from Vicugna pacos was enhanced, the sustitution of serine 102 with aspartic acid improved affinity by 3.3-fold compared to Nb28.

This sdAb secuence is prepared to fuse with a signal peptide and a cell wall protein for yeast surface display, this is the reason why it does not have either start or stop codon.

S. cerevisiae codon optimized.

Source

Modified sequence S102D from https://www.sdab-db.ca/?Display&ID=sdAb_3705_Vp

He, T., Nie, Y., Yan, T., Zhu, J., He, X., Li, Y., Zhang, Q., Tang, X., Hu, R., Yang, Y., & Liu, M. (2021). Enhancing the detection sensitivity of nanobody against aflatoxin B1 through structure-guided modification. International Journal Of Biological Macromolecules, 194, 188-197. https://doi.org/10.1016/j.ijbiomac.2021.11.182